› 5o2n

X-ray diffraction
1.51Å resolution

Lytic transglycosylase in action

Released:
DOI: 10.2210/pdb5o2n/pdb
PDB entry 5o2n coloured by chain, front view.
PDB entry 5o2n coloured by chain, side view.
PDB entry 5o2n coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
A step-by-step
Williams AH, Wheeler R, Rateau L, Malosse C, Chamot-Rooke J, Haouz A, Taha MK, Boneca IG
J Biol Chem 293 6000-6010 (2018)
PMID: 29483188

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Transglycosylase SLT domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 616 amino acids
Theoretical weight: 68.53 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9JXP1 (Residues: 1-616; Coverage: 100%)
Gene name: NMB1949
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand FLC 1 x FLC
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 67.1Å b: 72.6Å c: 125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.192
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
1 more

4 mentions without citation

A step-by-step in crystallo guide to bond cleavage and 1,6-anhydro-sugar product synthesis by a peptidoglycan-degrading lytic transglycosylase.
Williams et al. (2018)
3 more