› 5o3r

X-ray diffraction
1.9Å resolution

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with AMP

Released:
DOI: 10.2210/pdb5o3r/pdb
PDB entry 5o3r coloured by chain, front view.
PDB entry 5o3r coloured by chain, side view.
PDB entry 5o3r coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
PII-like signaling protein SbtB links cAMP sensing with cyanobacterial inorganic carbon response.
Selim KA, Haase F, Hartmann MD, Hagemann M, Forchhammer K
Proc Natl Acad Sci U S A 115 E4861-E4869 (2018)
PMID: 29735650

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-associated protein slr1513 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 120 amino acids
Theoretical weight: 13.22 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P73954 (Residues: 1-110; Coverage: 100%)
Gene name: slr1513
Sequence domains: Nitrogen regulatory protein P-II

Ligands and Environments

2 bound ligands:
Ligand AMP 3 x AMP
Ligand BCT 1 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P32
Unit cell:
a: 64.425Å b: 64.425Å c: 80.737Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.207
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns.
Rottet et al. (2021)
3 more

6 mentions without citation

Carbon/nitrogen homeostasis control in cyanobacteria.
Forchhammer et al. (2020)
5 more