› 5o63

X-ray diffraction
1.6Å resolution

Crystal structure of UbaLAI restriction endonuclease B3 domain domain (mutant L24M L53M L95M) with cognate DNA

Released:
DOI: 10.2210/pdb5o63/pdb
PDB entry 5o63 coloured by chain, front view.
PDB entry 5o63 coloured by chain, side view.
PDB entry 5o63 coloured by chain, top view.
Source organisms:
Primary publication:
UbaLAI is a monomeric Type IIE restriction enzyme.
Sasnauskas G, Tamulaitiene G, Tamulaitis G, Calyševa J, Laime M, Rimšeliene R, Lubys A, Siksnys V
OpenAccess logo Nucleic Acids Res 45 9583-9594 (2017)
PMID: 28934493

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-119081 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Restriction endonuclease UbaLAI Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 20.99 KDa
Source organism: unidentified
Expression system: Escherichia coli
DNA (5'-D(*C*G*C*C*A*G*G*G*C*)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 9 nucleotides
Theoretical weight: 2.73 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*G*C*C*C*T*G*G*C*G*)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 9 nucleotides
Theoretical weight: 2.74 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P212121
Unit cell:
a: 40.775Å b: 94.583Å c: 123.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.159 0.191
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 citation in other articles

The crystal structure of the Helicobacter pylori LlaJI.R1 N-terminal domain provides a model for site-specific DNA binding.
Hosford et al. (2018)
2 more

2 mentions without citation

Structure of the Guanidine III Riboswitch.
Huang et al. (2017)
1 more