› 5o7j

Solution NMR

Structural insights into the periplasmic sensor domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5o7j/pdb
PDB entry 5o7j coloured by chain, ensemble of 20 models, front view.
PDB entry 5o7j coloured by chain, ensemble of 20 models, side view.
PDB entry 5o7j coloured by chain, ensemble of 20 models, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural and functional insights into the periplasmic detector domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa.
Ali-Ahmad A, Fadel F, Sebban-Kreuzer C, Ba M, Pélissier GD, Bornet O, Guerlesquin F, Bourne Y, Bordi C, Vincent F
OpenAccess logo Sci Rep 7 11262 (2017)
PMID: 28900144

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-515376 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.38 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XD98 (Residues: 38-164; Coverage: 14%)
Gene names: PA0928, gacS
Sequence domains: Single cache domain 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Surface Sensing for Biofilm Formation in Pseudomonas aeruginosa.
Chang CY. (2017)
3 more

2 mentions without citation

The Escherichia coli two-component signal sensor BarA binds protonated acetate via a conserved hydrophobic-binding pocket.
Alvarez et al. (2021)
1 more