› 5o7k

X-ray diffraction
2.05Å resolution

Crystal structure of a single chain monellin mutant (Y65R) pH 2.0

Released:
DOI: 10.2210/pdb5o7k/pdb
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PDB entry 5o7k coloured by chain, front view.
PDB entry 5o7k coloured by chain, side view.
PDB entry 5o7k coloured by chain, top view.
Source organism: Dioscoreophyllum cumminsii
Primary publication:
pH driven fibrillar aggregation of the super-sweet protein Y65R-MNEI: A step-by-step structural analysis.
Pica A, Leone S, Di Girolamo R, Donnarumma F, Emendato A, Rega MF, Merlino A, Picone D
Biochim Biophys Acta Gen Subj 1862 808-815 (2018)
PMID: 29288772

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monellin chain A; Monellin chain B Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11.28 KDa
Source organism: Dioscoreophyllum cumminsii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P02882 (Residues: 1-48; Coverage: 96%)
  • Canonical: P02881 (Residues: 1-45; Coverage: 100%)
Sequence domains: Monellin

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 128.912Å b: 31.501Å c: 45.743Å
α: 90° β: 106.24° γ: 90°
R-values:
R R work R free
0.196 0.193 0.252
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

6 citation in other articles

Platinum(II) O,S Complexes Inhibit the Aggregation of Amyloid Model Systems.
Florio et al. (2019)
5 more

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