› 5o7s

X-ray diffraction
2.02Å resolution

Crystal structure of a single chain monellin mutant (Y65R) pH 8.3

Released:
DOI: 10.2210/pdb5o7s/pdb
PDB entry 5o7s coloured by chain, front view.
PDB entry 5o7s coloured by chain, side view.
PDB entry 5o7s coloured by chain, top view.
Source organism: Dioscoreophyllum cumminsii
Primary publication:
pH driven fibrillar aggregation of the super-sweet protein Y65R-MNEI: A step-by-step structural analysis.
Pica A, Leone S, Di Girolamo R, Donnarumma F, Emendato A, Rega MF, Merlino A, Picone D
Biochim Biophys Acta Gen Subj 1862 808-815 (2018)
PMID: 29288772

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monellin chain A; Monellin chain B Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11.28 KDa
Source organism: Dioscoreophyllum cumminsii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P02882 (Residues: 1-48; Coverage: 96%)
  • Canonical: P02881 (Residues: 1-45; Coverage: 100%)
Sequence domains: Monellin

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 35.25Å b: 37.43Å c: 45.74Å
α: 93.67° β: 112.64° γ: 115.12°
R-values:
R R work R free
0.241 0.238 0.287
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

6 citation in other articles

Platinum(II) O,S Complexes Inhibit the Aggregation of Amyloid Model Systems.
Florio et al. (2019)
5 more