› 5oay

Solution NMR

M. tuberculosis [4Fe-4S] protein WhiB1 is a four-helix bundle that forms a NO-sensitive complex with sigmaA and regulates the major virulence factor ESX-1

Released:
DOI: 10.2210/pdb5oay/pdb
PDB entry 5oay coloured by chain, ensemble of 10 models, front view.
PDB entry 5oay coloured by chain, ensemble of 10 models, side view.
PDB entry 5oay coloured by chain, ensemble of 10 models, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure of a Wbl protein and implications for NO sensing by M. tuberculosis.
Kudhair BK, Hounslow AM, Rolfe MD, Crack JC, Hunt DM, Buxton RS, Smith LJ, Le Brun NE, Williamson MP, Green J
OpenAccess logo Nat Commun 8 2280 (2017)
PMID: 29273788

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator WhiB1 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.38 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: P9WF43 (Residues: 1-84; Coverage: 100%)
Gene names: Rv3219, whiB1
Sequence domains: Transcription factor WhiB

Ligands and Environments

1 bound ligand:
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 65%
Refinement method: simulated annealing
Expression system: Mycolicibacterium smegmatis

Quick links

Citations

4 review citations

The NMR contribution to protein-protein networking in Fe-S protein maturation.
Banci et al. (2018)
3 more

3 mentions without citation

The actinobacterial WhiB-like (Wbl) family of transcription factors.
Bush MJ. (2018)
2 more