› 5obp

X-ray diffraction
1.59Å resolution

PCE reductive dehalogenase from S. multivorans with 6-hydroxybenzimidazolyl norcobamide cofactor

Released:
DOI: 10.2210/pdb5obp/pdb
PDB entry 5obp coloured by chain, front view.
PDB entry 5obp coloured by chain, side view.
PDB entry 5obp coloured by chain, top view.
Source organism: Sulfurospirillum multivorans
Primary publication:
Selective Utilization of Benzimidazolyl-Norcobamides as Cofactors by the Tetrachloroethene Reductive Dehalogenase of Sulfurospirillum multivorans.
Keller S, Kunze C, Bommer M, Paetz C, Menezes RC, Svatoš A, Dobbek H, Schubert T
J Bacteriol 200 (2018)
PMID: 29378885

Function and Biology Details

Reaction catalysed: 
Trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-521664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrachloroethene reductive dehalogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 52.16 KDa
Source organism: Sulfurospirillum multivorans
UniProt:
  • Canonical: O68252 (Residues: 38-501; Coverage: 100%)
Gene names: SMN_1516, pceA
Sequence domains: 4Fe-4S double cluster binding domain

Ligands and Environments

4 bound ligands:
Ligand SF4 4 x SF4
Ligand 9QQ 2 x 9QQ
Ligand BEN 1 x BEN
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P41
Unit cell:
a: 73.565Å b: 73.565Å c: 184.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.177

Quick links

Citations

1 review citation

Sharing vitamins: Cobamides unveil microbial interactions.
Sokolovskaya et al. (2020)
8 other articles

1 mention without citation

Selective Utilization of Benzimidazolyl-Norcobamides as Cofactors by the Tetrachloroethene Reductive Dehalogenase of Sulfurospirillum multivorans.
Keller et al. (2018)