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Thermus scotoductus SA-01 Ene-reductase triple mutant TsER_C25D_I67T_A102H

X-ray diffraction
2.3Å resolution
PDB entry 5ogt coloured by chain, front view.
PDB entry 5ogt coloured by chain, side view.
PDB entry 5ogt coloured by chain, top view.
Source organism: Thermus scotoductus SA-01
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 25692.48 Å2
Buried surface area: 4503.85 Å2
Dissociation area: 1,479.47 Å2
Dissociation energy (ΔGdiss): 7.34 kcal/mol
Dissociation entropy (TΔSdiss): 13.76 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-123113
Assembly 2
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 25769.03 Å2
Buried surface area: 4410.86 Å2
Dissociation area: 1,441.61 Å2
Dissociation energy (ΔGdiss): 7.36 kcal/mol
Dissociation entropy (TΔSdiss): 13.75 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-123113
Assembly 3
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 25773.75 Å2
Buried surface area: 4416.03 Å2
Dissociation area: 1,443.06 Å2
Dissociation energy (ΔGdiss): 7.42 kcal/mol
Dissociation entropy (TΔSdiss): 13.75 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-123113
Assembly 4
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Assembly Name: NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
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Multimeric state: homo dimer
Accessible surface area: 25774.58 Å2
Buried surface area: 4356.2 Å2
Dissociation area: 1,408.96 Å2
Dissociation energy (ΔGdiss): 10.15 kcal/mol
Dissociation entropy (TΔSdiss): 13.75 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-123113

Macromolecules

NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein
Chains: A, B, C, D
Length: 349 amino acids
Theoretical weight: 38.09 KDa
Source organism: Thermus scotoductus SA-01
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E8PRF1 (Residues: 1-347; Coverage: 100%)
Gene name: TSC_p800090
Pfam: NADH:flavin oxidoreductase / NADH oxidase family
InterPro:
CATH: Aldolase class I
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 5ogt, assembly 1, front view.
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 5ogt, assembly 1, side view.
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein in PDB entry 5ogt, assembly 1, top view.
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