› 5oj0

X-ray diffraction
2.66Å resolution

Penicillin-Binding Protein 2X (PBP2X) from Streptococcus pneumoniae in complex with Cefepime

Released:
DOI: 10.2210/pdb5oj0/pdb
PDB entry 5oj0 coloured by chain, front view.
PDB entry 5oj0 coloured by chain, side view.
PDB entry 5oj0 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Allostery, Recognition of Nascent Peptidoglycan, and Cross-linking of the Cell Wall by the Essential Penicillin-Binding Protein 2x of Streptococcus pneumoniae.
Bernardo-García N, Mahasenan KV, Batuecas MT, Lee M, Hesek D, Petráčková D, Doubravová L, Branny P, Mobashery S, Hermoso JA
ACS Chem Biol 13 694-702 (2018)
PMID: 29357220

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-493308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PASTA domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 76.81 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0E8T757 (Residues: 49-750; Coverage: 94%)
Gene names: GM539_01965, SAMEA3381574_01613, pbpX
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 9WT 1 x 9WT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P3121
Unit cell:
a: 99.547Å b: 99.547Å c: 189.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.172 0.231
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Constructing and deconstructing the bacterial cell wall.
Fisher et al. (2020)
15 other articles

1 mention without citation

In-vitro and in-silico antibacterial activity of Azadirachta indica (Neem), methanolic extract, and identification of Beta.d-Mannofuranoside as a promising antibacterial agent.
Altayb et al. (2022)