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X-ray diffraction
1.6Å resolution

Crystal structure of the dehydrogenase/reductase SDR family member 4 (DHRS4) from Caenorhabditis elegans

Released:
DOI: 10.2210/pdb5oji/pdb
PDB entry 5oji coloured by chain, front view.
PDB entry 5oji coloured by chain, side view.
PDB entry 5oji coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Crystal structure and catalytic characterization of the dehydrogenase/reductase SDR family member 4 (DHRS4) from Caenorhabditis elegans.
Kisiela M, Faust A, Ebert B, Maser E, Scheidig AJ
FEBS J 285 275-293 (2018)
PMID: 29151266

Function and Biology Details

Reaction catalysed: 
R-CHOH-R' + NADP(+) = R-CO-R' + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124529 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenase/reductase SDR family member 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 27.62 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G5EGA6 (Residues: 1-260; Coverage: 100%)
Gene names: F54F3.4, dhrs-4
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
Ligand ISN 2 x ISN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P42212
Unit cell:
a: 94.75Å b: 94.75Å c: 132.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.229
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

Genome-Wide Association Study Dissects the Genetic Architecture of Maize Husk Tightness.
Jiang et al. (2020)
4 more

1 mention without citation

Crystal structure and catalytic characterization of the dehydrogenase/reductase SDR family member 4 (DHRS4) from Caenorhabditis elegans.
Kisiela et al. (2018)