› 5olt

X-ray diffraction
1.45Å resolution

Crystal structure of the extramembrane domain of the cellulose biosynthetic protein BcsG from Salmonella typhimurium

Released:
DOI: 10.2210/pdb5olt/pdb
PDB entry 5olt coloured by chain, front view.
PDB entry 5olt coloured by chain, side view.
PDB entry 5olt coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Structural and Functional Characterization of the BcsG Subunit of the Cellulose Synthase in Salmonella typhimurium.
Sun L, Vella P, Schnell R, Polyakova A, Bourenkov G, Li F, Cimdins A, Schneider TR, Lindqvist Y, Galperin MY, Schneider G, Römling U
J Mol Biol 430 3170-3189 (2018)
PMID: 30017920

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulose biosynthesis protein BcsG Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 42.13 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q7CPI7 (Residues: 185-559; Coverage: 67%)
Gene names: STM3624, bcsG
Sequence domains: Cellulose biosynthesis protein BcsG

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P3121
Unit cell:
a: 80.658Å b: 80.658Å c: 97.673Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.128 0.127 0.157
Expression system: Escherichia coli DH5[alpha]

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Citations

4 review citations

Regulation of Biofilm Exopolysaccharide Production by Cyclic Di-Guanosine Monophosphate.
Poulin et al. (2021)
3 more

4 mentions without citation

Phosphoethanolamine Transferases as Drug Discovery Targets for Therapeutic Treatment of Multi-Drug Resistant Pathogenic Gram-Negative Bacteria.
Thai et al. (2023)
3 more