› 5oo5

X-ray diffraction
1.78Å resolution

Streptomyces PAC13 with uridine uronic acid

Released:
DOI: 10.2210/pdb5oo5/pdb
PDB entry 5oo5 coloured by chain, front view.
PDB entry 5oo5 coloured by chain, side view.
PDB entry 5oo5 coloured by chain, top view.
Source organism: Streptomyces coeruleorubidus
Primary publication:
Pac13 is a Small, Monomeric Dehydratase that Mediates the Formation of the 3'-Deoxy Nucleoside of Pacidamycins.
Michailidou F, Chung CW, Brown MJB, Bent AF, Naismith JH, Leavens WJ, Lynn SM, Sharma SV, Goss RJM
OpenAccess logo Angew Chem Int Ed Engl 56 12492-12497 (2017)
PMID: 28786545

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative cupin_2 domain-containing isomerase Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.11 KDa
Source organism: Streptomyces coeruleorubidus
Expression system: Escherichia coli
UniProt:
  • Canonical: E2EKP5 (Residues: 1-121; Coverage: 100%)
Gene names: pac13, pacM

Ligands and Environments

1 bound ligand:
Ligand UUA 1 x UUA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P3221
Unit cell:
a: 66.489Å b: 66.489Å c: 55.232Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.153 0.195
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Identification and characterization of enzymes involved in the biosynthesis of pyrimidine nucleoside antibiotics.
McErlean et al. (2021)
5 other articles

1 mention without citation

Pac13 is a Small, Monomeric Dehydratase that Mediates the Formation of the 3'-Deoxy Nucleoside of Pacidamycins.
Michailidou et al. (2017)