› 5oqe

X-ray diffraction
1.91Å resolution

XFEL structure of the on state of a reversibly photoswitching fluorescent protein determined using the droplet injection method

Released:
DOI: 10.2210/pdb5oqe/pdb
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PDB entry 5oqe coloured by chain, front view.
PDB entry 5oqe coloured by chain, side view.
PDB entry 5oqe coloured by chain, top view.
Source organism: Lobophyllia hemprichii
Primary publication:
X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature.
Hutchison CDM, Cordon-Preciado V, Morgan RML, Nakane T, Ferreira J, Dorlhiac G, Sanchez-Gonzalez A, Johnson AS, Fitzpatrick A, Fare C, Marangos JP, Yoon CH, Hunter MS, DePonte DP, Boutet S, Owada S, Tanaka R, Tono K, Iwata S, van Thor JJ
OpenAccess logo Int J Mol Sci 18 (2017)
PMID: 28880248

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green to red photoconvertible GFP-like protein EosFP Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.59 KDa
Source organism: Lobophyllia hemprichii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5S6Z9 (Residues: 2-226; Coverage: 100%)
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand VYA 1 x VYA

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SACLA BEAMLINE BL3
Spacegroup: P212121
Unit cell:
a: 39.8Å b: 74.6Å c: 79.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.167 0.241
Expression system: Escherichia coli BL21(DE3)

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Citations

7 citation in other articles

Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography.
Olmos et al. (2018)
6 more

1 mention without citation

X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature.
Hutchison et al. (2017)

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