› 5ou5

X-ray diffraction
2.2Å resolution

Crystal structure of maize chloroplastic photosynthetic NADP(+)-dependent malic enzyme

Released:
DOI: 10.2210/pdb5ou5/pdb
PDB entry 5ou5 coloured by chain, front view.
PDB entry 5ou5 coloured by chain, side view.
PDB entry 5ou5 coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Molecular adaptations of NADP-malic enzyme for its function in C4 photosynthesis in grasses.
Alvarez CE, Bovdilova A, Höppner A, Wolff CC, Saigo M, Trajtenberg F, Zhang T, Buschiazzo A, Nagel-Steger L, Drincovich MF, Lercher MJ, Maurino VG
Nat Plants 5 755-765 (2019)
PMID: 31235877

Function and Biology Details

Reaction catalysed: 
(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent malic enzyme, chloroplastic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 576 amino acids
Theoretical weight: 63.64 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P16243 (Residues: 62-636; Coverage: 90%)
Gene names: ME1, MOD1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NA 16 x NA
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I212121
Unit cell:
a: 135.961Å b: 147.155Å c: 261.615Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.211
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Role of C4 photosynthetic enzyme isoforms in C3 plants and their potential applications in improving agronomic traits in crops.
Singh et al. (2022)
8 other articles

2 mentions without citation

Structural and Molecular Dynamics of Mycobacterium tuberculosis Malic Enzyme, a Potential Anti-TB Drug Target.
Burley et al. (2021)
1 more