› 5pwd

X-ray diffraction
1.57Å resolution

PanDDA analysis group deposition -- Crystal Structure of SP100 in complex with N09600b

Released:
DOI: 10.2210/pdb5pwd/pdb
PDB entry 5pwd coloured by chain, front view.
PDB entry 5pwd coloured by chain, side view.
PDB entry 5pwd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A multi-crystal method for extracting obscured crystallographic states from conventionally uninterpretable electron density.
Pearce NM, Krojer T, Bradley AR, Collins P, Nowak RP, Talon R, Marsden BD, Kelm S, Shi J, Deane CM, von Delft F
OpenAccess logo Nat Commun 8 15123 (2017)
PMID: 28436492

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SP100 nuclear antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 21.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: H0Y4R8 (Residues: 74-253; Coverage: 70%)
Gene name: SP100
Sequence domains: Bromodomain
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand MES 1 x MES
Ligand EDO 2 x EDO
Ligand CL 2 x CL
Ligand 81P 1 x 81P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 127.688Å b: 45.389Å c: 83.411Å
α: 90° β: 102.16° γ: 90°
R-values:
R R work R free
0.181 0.18 0.21
Expression system: Escherichia coli

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Citations

15 review citations

Structural Biology and the Design of New Therapeutics: From HIV and Cancer to Mycobacterial Infections: A Paper Dedicated to John Kendrew.
Thomas et al. (2017)
14 more