› 5r4d

X-ray diffraction
1.05Å resolution

Crystal Structure of gamma-Chymotrypsin at pH 9, cryo temperature

Released:
DOI: 10.2210/pdb5r4d/pdb
PDB entry 5r4d coloured by chain, front view.
PDB entry 5r4d coloured by chain, side view.
PDB entry 5r4d coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Kreinbring CA, Wilson MA, Kovalevsky AY, Blakeley MP, Fisher SZ, Lazar LM, Moulin AG, Novak WR, Petsko GA, Ringe D

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133424 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Chymotrypsin A chain A Chain: A
Molecule details ›
Chain: A
Length: 13 amino acids
Theoretical weight: 1.25 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00766 (Residues: 1-13; Coverage: 5%)
Chymotrypsin A chain B Chain: B
Molecule details ›
Chain: B
Length: 131 amino acids
Theoretical weight: 13.93 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00766 (Residues: 16-146; Coverage: 54%)
Sequence domains: Trypsin
Chymotrypsin A chain C Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 10.07 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00766 (Residues: 149-245; Coverage: 40%)
Sequence domains: Trypsin
peptide GSWPW Chain: D
Molecule details ›
Chain: D
Length: 5 amino acids
Theoretical weight: 632 Da
Source organism: Bos taurus
peptide TPGVY Chain: E
Molecule details ›
Chain: E
Length: 5 amino acids
Theoretical weight: 536 Da
Source organism: Bos taurus

Ligands and Environments

2 bound ligands:
Ligand IOD 2 x IOD
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P42212
Unit cell:
a: 69.067Å b: 69.067Å c: 95.803Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.122 0.14

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