› 5rpg

X-ray diffraction
1.5Å resolution

PanDDA analysis group deposition -- Proteinase K changed state model for fragment Frag Xtal Screen H2a

Released:
DOI: 10.2210/pdb5rpg/pdb
PDB entry 5rpg coloured by chain, front view.
PDB entry 5rpg coloured by chain, side view.
PDB entry 5rpg coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
FragMAXapp: crystallographic fragment-screening data-analysis and project-management system.
Lima GMA, Jagudin E, Talibov VO, Benz LS, Marullo C, Barthel T, Wollenhaupt J, Weiss MS, Mueller U
OpenAccess logo Acta Crystallogr D Struct Biol 77 799-808 (2021)
PMID: 34076593

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.96 KDa
Source organism: Parengyodontium album
Expression system: Escherichia coli
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand AMH 1 x AMH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P43212
Unit cell:
a: 67.96Å b: 67.96Å c: 102.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.158
Expression system: Escherichia coli

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Citations

1 review citation

A Crystallographic Snapshot of SARS-CoV-2 Main Protease Maturation Process.
Noske et al. (2021)
7 other articles