› 5s4v

X-ray diffraction
2.3Å resolution

Tubulin-Z57040482-complex

Released:
DOI: 10.2210/pdb5s4v/pdb
Model geometry
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PDB entry 5s4v coloured by chain, front view.
PDB entry 5s4v coloured by chain, side view.
PDB entry 5s4v coloured by chain, top view.
Source organisms:
Primary publication:
Comprehensive Analysis of Binding Sites in Tubulin.
Mühlethaler T, Gioia D, Prota AE, Sharpe ME, Cavalli A, Steinmetz MO
OpenAccess logo Angew Chem Int Ed Engl 60 13331-13342 (2021)
PMID: 33951246

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-237092 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Tubulin-Tyrosine Ligase Chain: F

Ligands and Environments

7 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 5 x MG
Ligand CA 4 x CA
Ligand GDP 2 x GDP
Ligand MES 1 x MES
Ligand 9KS 2 x 9KS
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 104.42Å b: 158.06Å c: 178.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.242
Expression system: Escherichia coli

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Citations

7 review citations

Molecular interactions at the colchicine binding site in tubulin: An X-ray crystallography perspective.
Wang et al. (2022)
6 more

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