› 5t1j

X-ray diffraction
2.95Å resolution

Crystal Structure of the Tbox DNA binding domain of the transcription factor T-bet

Released:
DOI: 10.2210/pdb5t1j/pdb
PDB entry 5t1j coloured by chain, front view.
PDB entry 5t1j coloured by chain, side view.
PDB entry 5t1j coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the DNA binding domain of the transcription factor T-bet suggests simultaneous recognition of distant genome sites.
Liu CF, Brandt GS, Hoang QQ, Naumova N, Lazarevic V, Hwang ES, Dekker J, Glimcher LH, Ringe D, Petsko GA
Proc Natl Acad Sci U S A 113 E6572-E6581 (2016)
PMID: 27791029

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119131 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
T-box transcription factor TBX21 Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 23.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JKD8 (Residues: 135-326; Coverage: 36%)
Gene names: Tbet, Tblym, Tbx21
Sequence domains: T-box
Structure domains: Transcription factor, T-box
DNA Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P61
Unit cell:
a: 70.45Å b: 70.45Å c: 438.389Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.267 0.266 0.294
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Transcription tipping points for T follicular helper cell and T-helper 1 cell fate commitment.
Sheikh et al. (2021)
4 more

4 mentions without citation

Crystal structure of the DNA binding domain of the transcription factor T-bet suggests simultaneous recognition of distant genome sites.
Liu et al. (2016)
3 more