› 5t1n

Solution NMR

Solution-state NMR structural ensemble of NPr (1-85) refined with RDCs and PCS

Released:
DOI: 10.2210/pdb5t1n/pdb
PDB entry 5t1n coloured by chain, ensemble of 20 models, front view.
PDB entry 5t1n coloured by chain, ensemble of 20 models, side view.
PDB entry 5t1n coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Structure of the NPr:EINNtr Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.
Strickland M, Stanley AM, Wang G, Botos I, Schwieters CD, Buchanan SK, Peterkofsky A, Tjandra N
Structure 24 2127-2137 (2016)
PMID: 27839951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphocarrier protein NPr Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.25 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9N0 (Residues: 1-85; Coverage: 94%)
Gene names: JW3173, b3206, npr, ptsO, rpoR, yhbK
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 32%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

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Citations

1 review citation

Sophisticated Regulation of Transcriptional Factors by the Bacterial Phosphoenolpyruvate: Sugar Phosphotransferase System.
Galinier et al. (2017)
8 other articles

3 mentions without citation

Integrative Protein Modeling in RosettaNMR from Sparse Paramagnetic Restraints.
Kuenze et al. (2019)
2 more