› 5t3m

Solution NMR

Solution structure of a triple mutant of HwTx-IV - a potent blocker of Nav1.7

Released:
DOI: 10.2210/pdb5t3m/pdb
PDB entry 5t3m coloured by chain, ensemble of 20 models, front view.
PDB entry 5t3m coloured by chain, ensemble of 20 models, side view.
PDB entry 5t3m coloured by chain, ensemble of 20 models, top view.
Source organism: Cyriopagopus schmidti
Primary publication:
The structure, dynamics and selectivity profile of a NaV1.7 potency-optimised huwentoxin-IV variant.
Rahnama S, Deuis JR, Cardoso FC, Ramanujam V, Lewis RJ, Rash LD, King GF, Vetter I, Mobli M
OpenAccess logo PLoS One 12 e0173551 (2017)
PMID: 28301520

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Huwentoxin-IV Chain: A
Molecule details ›
Chain: A
Length: 35 amino acids
Theoretical weight: 4 KDa
Source organism: Cyriopagopus schmidti
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P83303 (Residues: 53-87; Coverage: 54%)
Sequence domains: Ion channel inhibitory toxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Structure-Function and Therapeutic Potential of Spider Venom-Derived Cysteine Knot Peptides Targeting Sodium Channels.
Cardoso et al. (2019)
7 more

6 mentions without citation

Structural Basis for High-Affinity Trapping of the NaV1.7 Channel in Its Resting State by Tarantula Toxin.
Wisedchaisri et al. (2021)
5 more