› 5t5d

X-ray diffraction
2.5Å resolution

Crystal Structure of the PTS IIB protein associated with the fucose utilization operon from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb5t5d/pdb
PDB entry 5t5d coloured by chain, front view.
PDB entry 5t5d coloured by chain, side view.
PDB entry 5t5d coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
Structural characterization of the PTS IIA and IIB proteins associated with pneumococcal fucose utilization.
Higgins MA, Hamilton AM, Boraston AB
Proteins 85 963-968 (2017)
PMID: 28168775

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS EIIB type-4 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.51 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2USH8 (Residues: 2-156; Coverage: 99%)
Gene name: SP_2163
Sequence domains: PTS system sorbose subfamily IIB component
Structure domains: Phosphotransferase system, sorbose subfamily IIB component

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6522
Unit cell:
a: 42.503Å b: 42.503Å c: 292.922Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.224 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
4 other articles