› 5t7o

X-ray diffraction
1.8Å resolution

Crystal structure of Trypanosoma cruzi Dihydrofolate Reductase-Thymidylate Synthase in complex with (6S)-5,6,7,8-TETRAHYDROFOLATE

Released:
DOI: 10.2210/pdb5t7o/pdb
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PDB entry 5t7o coloured by chain, front view.
PDB entry 5t7o coloured by chain, side view.
PDB entry 5t7o coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Entry authors: Di Pisa F, Dello Iacono L, Bonucci A, Mangani S

Function and Biology Details

Reactions catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional dihydrofolate reductase-thymidylate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 521 amino acids
Theoretical weight: 58.93 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4DLS1 (Residues: 1-521; Coverage: 100%)
Gene name: Tc00.1047053509153.90
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP

Cofactor: Ligand THG 4 x THG
2 bound ligands:
Ligand UMP 4 x UMP
Ligand EDO 47 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: I222
Unit cell:
a: 154.547Å b: 173.54Å c: 174.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.22
Expression system: Escherichia coli BL21(DE3)

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