› 5t8t

X-ray diffraction
2.1Å resolution

Crystal Structure of a S-adenosylmethionine Synthase from Neisseria gonorrhoeae with bound AMP and Magnesium

Released:
DOI: 10.2210/pdb5t8t/pdb
PDB entry 5t8t coloured by chain, front view.
PDB entry 5t8t coloured by chain, side view.
PDB entry 5t8t coloured by chain, top view.
Source organism: Neisseria gonorrhoeae FA 1090
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 43.02 KDa
Source organism: Neisseria gonorrhoeae FA 1090
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5FAC0 (Residues: 1-389; Coverage: 100%)
Gene names: NGO_0106, metK
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

2 bound ligands:
Ligand AMP 1 x AMP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P4122
Unit cell:
a: 116.58Å b: 116.58Å c: 145.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.206
Expression system: Escherichia coli BL21(DE3)

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