› 5tbd

X-ray diffraction
2.2Å resolution

Crystal Structure of anti-MSP2 Fv fragment (mAb4D11) in complex with 3D7-MSP2 215-222

Released:
DOI: 10.2210/pdb5tbd/pdb
PDB entry 5tbd coloured by chain, front view.
PDB entry 5tbd coloured by chain, side view.
PDB entry 5tbd coloured by chain, top view.
Source organisms:
Primary publication:
Structure and Characterisation of a Key Epitope in the Conserved C-Terminal Domain of the Malaria Vaccine Candidate MSP2.
Seow J, Morales RA, MacRaild CA, Krishnarjuna B, McGowan S, Dingjan T, Jaipuria G, Rouet R, Wilde KL, Atreya HS, Richards JS, Anders RF, Christ D, Drinkwater N, Norton RS
J Mol Biol 429 836-846 (2017)
PMID: 28189425

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-546552 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fv fragment (mAb4D1) heavy chain Chains: A, D, E, J
Molecule details ›
Chains: A, D, E, J
Length: 114 amino acids
Theoretical weight: 12.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fv fragment (mAb4D1) heavy chain Chains: B, F, G, K
Molecule details ›
Chains: B, F, G, K
Length: 112 amino acids
Theoretical weight: 12.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Merozoite surface protein 2 Chains: C, H, I, L
Molecule details ›
Chains: C, H, I, L
Length: 10 amino acids
Theoretical weight: 831 Da
Source organism: Plasmodium falciparum 3D7
Expression system: Not provided
UniProt:
  • Canonical: P50498 (Residues: 233-240; Coverage: 3%)
Gene names: MSA2, MSP2, PF3D7_0206800, PFB0300c

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P1
Unit cell:
a: 41.975Å b: 72.97Å c: 81.965Å
α: 68.19° β: 78.75° γ: 73.83°
R-values:
R R work R free
0.209 0.207 0.247
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 citation in other articles

Lipid interactions modulate the structural and antigenic properties of the C-terminal domain of the malaria antigen merozoite surface protein 2.
Das et al. (2017)
2 more

2 mentions without citation

Disordered epitopes as peptide vaccines.
MacRaild et al. (2018)
1 more