› 5tcu

Electron Microscopy
3.9Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 52-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-249546 (preferred)
Entry contents:
46 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (52 distinct):
Small ribosomal subunit protein uS3 Chain: SB
Molecule details ›
Chain: SB
Length: 202 amino acids
Theoretical weight: 22.68 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW12 (Residues: 2-203; Coverage: 93%)
Gene names: SAOUHSC_02506, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: SC
Molecule details ›
Chain: SC
Length: 198 amino acids
Theoretical weight: 22.85 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FXK6 (Residues: 3-200; Coverage: 99%)
Gene names: SAOUHSC_01829, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: SD
Molecule details ›
Chain: SD
Length: 156 amino acids
Theoretical weight: 16.52 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW23 (Residues: 10-165; Coverage: 94%)
Gene names: SAOUHSC_02494, rpsE
Sequence domains:
Small ribosomal subunit protein bS6 Chain: SE
Molecule details ›
Chain: SE
Length: 95 amino acids
Theoretical weight: 11.24 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2G113 (Residues: 1-95; Coverage: 97%)
Gene names: SAOUHSC_00348, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: SG
Molecule details ›
Chain: SG
Length: 155 amino acids
Theoretical weight: 17.7 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: P48940 (Residues: 2-156; Coverage: 99%)
Gene names: SAOUHSC_00528, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: SF
Molecule details ›
Chain: SF
Length: 130 amino acids
Theoretical weight: 14.62 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW20 (Residues: 3-132; Coverage: 99%)
Gene names: SAOUHSC_02498, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: SH
Molecule details ›
Chain: SH
Length: 127 amino acids
Theoretical weight: 14.31 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW39 (Residues: 6-132; Coverage: 96%)
Gene names: SAOUHSC_02477, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: S1
Molecule details ›
Chain: S1
Length: 80 amino acids
Theoretical weight: 9.24 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2YYP5 (Residues: 6-85; Coverage: 78%)
Gene names: SAB2123c, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: S2
Molecule details ›
Chain: S2
Length: 114 amino acids
Theoretical weight: 12.05 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW31 (Residues: 15-128; Coverage: 88%)
Gene names: SAOUHSC_02486, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: S3
Molecule details ›
Chain: S3
Length: 136 amino acids
Theoretical weight: 15.19 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: P0A0H0 (Residues: 2-137; Coverage: 99%)
Gene names: SAOUHSC_00527, rpsL
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: S4
Molecule details ›
Chain: S4
Length: 113 amino acids
Theoretical weight: 12.84 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW30 (Residues: 2-114; Coverage: 93%)
Gene names: SAOUHSC_02487, rpsM
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS14B Chain: S5
Molecule details ›
Chain: S5
Length: 60 amino acids
Theoretical weight: 7.19 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW19 (Residues: 2-61; Coverage: 98%)
Gene names: SAOUHSC_02499, rpsN1, rpsZ
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: S6
Molecule details ›
Chain: S6
Length: 88 amino acids
Theoretical weight: 10.5 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2G2Q1 (Residues: 2-89; Coverage: 99%)
Gene names: SAOUHSC_01250, rpsO
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: S7
Molecule details ›
Chain: S7
Length: 83 amino acids
Theoretical weight: 9.29 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FZ45 (Residues: 2-84; Coverage: 91%)
Gene names: SAOUHSC_01208, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: S8
Molecule details ›
Chain: S8
Length: 80 amino acids
Theoretical weight: 9.37 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW15 (Residues: 5-84; Coverage: 92%)
Gene names: SAOUHSC_02503, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS18 Chain: S9
Molecule details ›
Chain: S9
Length: 56 amino acids
Theoretical weight: 6.57 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2G111 (Residues: 22-77; Coverage: 70%)
Gene names: SAOUHSC_00350, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein uS19 Chain: SI
Molecule details ›
Chain: SI
Length: 78 amino acids
Theoretical weight: 9.01 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW10 (Residues: 4-81; Coverage: 85%)
Gene names: SAOUHSC_02508, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein bS20 Chain: SA
Molecule details ›
Chain: SA
Length: 79 amino acids
Theoretical weight: 8.61 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FXY6 (Residues: 5-83; Coverage: 95%)
Gene names: SAOUHSC_01689, rpsT
Sequence domains: Ribosomal protein S20
Large ribosomal subunit protein uL2 Chain: L2
Molecule details ›
Chain: L2
Length: 274 amino acids
Theoretical weight: 29.83 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: P60430 (Residues: 2-275; Coverage: 99%)
Gene names: SAOUHSC_02509, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: LC
Molecule details ›
Chain: LC
Length: 215 amino acids
Theoretical weight: 23.2 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW06 (Residues: 2-216; Coverage: 98%)
Gene names: SAOUHSC_02512, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: LJ
Molecule details ›
Chain: LJ
Length: 205 amino acids
Theoretical weight: 22.29 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW07 (Residues: 3-207; Coverage: 99%)
Gene names: SAOUHSC_02511, rplD
Sequence domains: Ribosomal protein L4/L1 family
Large ribosomal subunit protein uL5 Chain: LK
Molecule details ›
Chain: LK
Length: 166 amino acids
Theoretical weight: 18.76 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW18 (Residues: 2-167; Coverage: 93%)
Gene names: SAOUHSC_02500, rplE
Sequence domains:
Large ribosomal subunit protein uL6 Chain: LL
Molecule details ›
Chain: LL
Length: 174 amino acids
Theoretical weight: 19.4 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW21 (Residues: 2-175; Coverage: 98%)
Gene names: SAOUHSC_02496, rplF
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein uL13 Chain: LM
Molecule details ›
Chain: LM
Length: 145 amino acids
Theoretical weight: 16.36 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW38 (Residues: 1-145; Coverage: 100%)
Gene names: SAOUHSC_02478, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL14 Chain: LN
Molecule details ›
Chain: LN
Length: 122 amino acids
Theoretical weight: 13.16 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW16 (Residues: 1-122; Coverage: 100%)
Gene names: SAOUHSC_02502, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: LO
Molecule details ›
Chain: LO
Length: 145 amino acids
Theoretical weight: 15.52 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: P0A0F8 (Residues: 1-145; Coverage: 99%)
Gene names: SAOUHSC_02492, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: LP
Molecule details ›
Chain: LP
Length: 136 amino acids
Theoretical weight: 15.49 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW13 (Residues: 1-136; Coverage: 94%)
Gene names: SAOUHSC_02505, rplP
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein bL17 Chain: LQ
Molecule details ›
Chain: LQ
Length: 119 amino acids
Theoretical weight: 13.42 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW33 (Residues: 4-122; Coverage: 98%)
Gene names: SAOUHSC_02484, rplQ
Sequence domains: Ribosomal protein L17
Large ribosomal subunit protein uL18 Chain: LR
Molecule details ›
Chain: LR
Length: 113 amino acids
Theoretical weight: 12.46 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW22 (Residues: 1-113; Coverage: 95%)
Gene names: SAOUHSC_02495, rplR
Sequence domains: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Large ribosomal subunit protein bL19 Chain: L1
Molecule details ›
Chain: L1
Length: 109 amino acids
Theoretical weight: 12.46 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FZ42 (Residues: 2-110; Coverage: 94%)
Gene names: SAOUHSC_01211, rplS
Sequence domains: Ribosomal protein L19
Large ribosomal subunit protein bL20 Chain: L3
Molecule details ›
Chain: L3
Length: 116 amino acids
Theoretical weight: 13.46 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FXQ1 (Residues: 2-117; Coverage: 98%)
Gene names: SAOUHSC_01784, rplT
Sequence domains: Ribosomal protein L20
Large ribosomal subunit protein bL21 Chain: L4
Molecule details ›
Chain: L4
Length: 102 amino acids
Theoretical weight: 11.35 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FXS8 (Residues: 1-102; Coverage: 100%)
Gene names: SAOUHSC_01757, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
Large ribosomal subunit protein uL22 Chain: L5
Molecule details ›
Chain: L5
Length: 112 amino acids
Theoretical weight: 12.33 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW11 (Residues: 1-112; Coverage: 96%)
Gene names: SAOUHSC_02507, rplV
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL23 Chain: L6
Molecule details ›
Chain: L6
Length: 89 amino acids
Theoretical weight: 10.38 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW08 (Residues: 2-90; Coverage: 98%)
Gene names: SAOUHSC_02510, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: L7
Molecule details ›
Chain: L7
Length: 103 amino acids
Theoretical weight: 11.32 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW17 (Residues: 2-104; Coverage: 98%)
Gene names: SAOUHSC_02501, rplX
Sequence domains:
Large ribosomal subunit protein bL25 Chain: L8
Molecule details ›
Chain: L8
Length: 93 amino acids
Theoretical weight: 10.39 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2G0S0 (Residues: 3-95; Coverage: 43%)
Gene names: SAOUHSC_00474, ctc, rplY
Sequence domains: Ribosomal L25p family
Large ribosomal subunit protein bL27 Chain: L9
Molecule details ›
Chain: L9
Length: 82 amino acids
Theoretical weight: 8.91 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FXT0 (Residues: 12-93; Coverage: 87%)
Gene names: SAOUHSC_01755, rpmA
Sequence domains: Ribosomal L27 protein
Large ribosomal subunit protein bL28 Chain: LA
Molecule details ›
Chain: LA
Length: 58 amino acids
Theoretical weight: 6.55 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FZ60 (Residues: 3-60; Coverage: 94%)
Gene names: SAOUHSC_01191, rpmB
Sequence domains: Ribosomal L28 family
Large ribosomal subunit protein uL29 Chain: LB
Molecule details ›
Chain: LB
Length: 62 amino acids
Theoretical weight: 7.33 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW14 (Residues: 5-66; Coverage: 90%)
Gene names: SAOUHSC_02504, rpmC
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein uL30 Chain: LD
Molecule details ›
Chain: LD
Length: 57 amino acids
Theoretical weight: 6.31 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: P0A0G2 (Residues: 2-58; Coverage: 97%)
Gene names: SAOUHSC_02493, rpmD
Sequence domains: Ribosomal protein L30p/L7e
Large ribosomal subunit protein bL32 Chain: LE
Molecule details ›
Chain: LE
Length: 47 amino acids
Theoretical weight: 5.41 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: A7X145 (Residues: 2-48; Coverage: 83%)
Gene names: SAHV_1119, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL33A Chain: LS
Molecule details ›
Chain: LS
Length: 47 amino acids
Theoretical weight: 5.68 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FYU6 (Residues: 2-48; Coverage: 96%)
Gene names: SAOUHSC_01328, rpmG1
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: LG
Molecule details ›
Chain: LG
Length: 43 amino acids
Theoretical weight: 5.25 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FUQ0 (Residues: 2-44; Coverage: 96%)
Gene names: SAOUHSC_03055, rpmH
Sequence domains: Ribosomal protein L34
Large ribosomal subunit protein bL35 Chain: LH
Molecule details ›
Chain: LH
Length: 60 amino acids
Theoretical weight: 6.99 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FXQ0 (Residues: 2-61; Coverage: 91%)
Gene names: SAOUHSC_01785, rpmI
Sequence domains: Ribosomal protein L35
Large ribosomal subunit protein bL36 Chain: LI
Molecule details ›
Chain: LI
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FW29 (Residues: 1-37; Coverage: 100%)
Gene names: SAOUHSC_02488, rpmJ
Sequence domains: Ribosomal protein L36
Large ribosomal subunit protein bL31B Chain: LF
Molecule details ›
Chain: LF
Length: 74 amino acids
Theoretical weight: 8.6 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
UniProt:
  • Canonical: Q2FWD8 (Residues: 1-74; Coverage: 88%)
Gene names: SAOUHSC_02361, rpmE2
Sequence domains: Ribosomal protein L31
16S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1555 nucleotides
Theoretical weight: 502.94 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
Messenger RNA Chain: X
Molecule details ›
Chain: X
Length: 13 nucleotides
Theoretical weight: 4.16 KDa
P-site tRNA CHAIN Chain: E
Molecule details ›
Chain: E
Length: 75 nucleotides
Theoretical weight: 24.16 KDa
Sequence domains: tRNA
E-site tRNA CHAIN Chain: D
Molecule details ›
Chain: D
Length: 76 nucleotides
Theoretical weight: 24.49 KDa
Sequence domains: tRNA
23S ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 2923 nucleotides
Theoretical weight: 946.69 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
5S rRNA CHAIN Chain: C
Molecule details ›
Chain: C
Length: 114 nucleotides
Theoretical weight: 36.69 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

2 bound ligands:
Ligand PAR 1 x PAR
Ligand MG 129 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-8402

Quick links

Citations

5 review citations

Linezolid: a review of its properties, function, and use in critical care.
Hashemian et al. (2018)
4 more

7 mentions without citation

Structural Basis for Linezolid Binding Site Rearrangement in the Staphylococcus aureus Ribosome.
Belousoff et al. (2017)
6 more