› 5tf0

X-ray diffraction
2.2Å resolution

Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis

Released:
DOI: 10.2210/pdb5tf0/pdb
PDB entry 5tf0 coloured by chain, front view.
PDB entry 5tf0 coloured by chain, side view.
PDB entry 5tf0 coloured by chain, top view.
Source organism: Bacteroides intestinalis DSM 17393
Entry authors: Kim Y, Hatzos-Skintges C, Endres M, Babnigg G, Joachimiak A, MCSG, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 751 amino acids
Theoretical weight: 83.96 KDa
Source organism: Bacteroides intestinalis DSM 17393
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3C777 (Residues: 26-773; Coverage: 100%)
Gene name: BACINT_00768
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand EDO 5 x EDO
1 modified residue:
Ligand MSE 54 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 80.57Å b: 88.447Å c: 107.282Å
α: 90° β: 97.89° γ: 90°
R-values:
R R work R free
0.217 0.215 0.264
Expression system: Escherichia coli BL21(DE3)

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