› 5tfq

X-ray diffraction
1.07Å resolution

Crystal structure of a representative of class A beta-lactamase from Bacteroides cellulosilyticus DSM 14838

Released:
DOI: 10.2210/pdb5tfq/pdb
PDB entry 5tfq coloured by chain, front view.
PDB entry 5tfq coloured by chain, side view.
PDB entry 5tfq coloured by chain, top view.
Source organism: Bacteroides cellulosilyticus DSM 14838
Entry authors: Nocek B, Hatzos-Skintges C, Babnigg G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 31.08 KDa
Source organism: Bacteroides cellulosilyticus DSM 14838
Expression system: Escherichia coli
UniProt:
  • Canonical: E2NI36 (Residues: 22-301; Coverage: 100%)
Gene name: BACCELL_03967
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 55.837Å b: 62.808Å c: 87.058Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.116 0.115 0.134
Expression system: Escherichia coli

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