› 5thk

X-ray diffraction
1.4Å resolution

Crystal Structure of a Putative Dehydrogenase from Burkholderia cenocepacia with bound NADP

Released:
DOI: 10.2210/pdb5thk/pdb
PDB entry 5thk coloured by chain, front view.
PDB entry 5thk coloured by chain, side view.
PDB entry 5thk coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-502780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 266 amino acids
Theoretical weight: 28.3 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B4E8W7 (Residues: 2-257; Coverage: 100%)
Gene name: BCAL2694
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand NAP 8 x NAP
Ligand MG 2 x MG
Ligand 1PE 2 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 75.54Å b: 102.44Å c: 122.25Å
α: 90° β: 97.28° γ: 90°
R-values:
R R work R free
0.14 0.14 0.164
Expression system: Escherichia coli BL21(DE3)

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