› 5tmx

Solution NMR

Solution Structure of SinI, antagonist to the master biofilm-regulator SinR in Bacillus subtilis

Released:
DOI: 10.2210/pdb5tmx/pdb
PDB entry 5tmx coloured by chain, ensemble of 10 models, front view.
PDB entry 5tmx coloured by chain, ensemble of 10 models, side view.
PDB entry 5tmx coloured by chain, ensemble of 10 models, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis.
Milton ME, Draughn GL, Bobay BG, Stowe SD, Olson AL, Feldmann EA, Thompson RJ, Myers KH, Santoro MT, Kearns DB, Cavanagh J
J Mol Biol 432 343-357 (2020)
PMID: 31493408

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein SinI Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.18 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23308 (Residues: 1-57; Coverage: 100%)
Gene names: BSU24600, sinI
Sequence domains: Anti-repressor SinI

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 47%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Bacillus subtilis biofilm formation and social interactions.
Arnaouteli et al. (2021)
2 more

1 mention without citation

The Solution Structures and Interaction of SinR and SinI: Elucidating the Mechanism of Action of the Master Regulator Switch for Biofilm Formation in Bacillus subtilis.
Milton et al. (2020)