› 5tnx

X-ray diffraction
1.7Å resolution

Crystal structure of Alcohol dehydrogenase zinc-binding domain protein from Burkholderia ambifaria

Released:
DOI: 10.2210/pdb5tnx/pdb
PDB entry 5tnx coloured by chain, front view.
PDB entry 5tnx coloured by chain, side view.
PDB entry 5tnx coloured by chain, top view.
Source organism: Burkholderia ambifaria MC40-6
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-502279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alcohol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 39.7 KDa
Source organism: Burkholderia ambifaria MC40-6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1Z4S6 (Residues: 1-375; Coverage: 100%)
Gene name: BamMC406_6306
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CIT 1 x CIT
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I222
Unit cell:
a: 79.06Å b: 92.65Å c: 120.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.172
Expression system: Escherichia coli BL21(DE3)

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