› 5to2

X-ray diffraction
1.65Å resolution

Crystal structure of streptavidin with one wild type subunit and three mutated subunits (N23A/S27D/S45A)

Released:
DOI: 10.2210/pdb5to2/pdb
PDB entry 5to2 coloured by chain, front view.
PDB entry 5to2 coloured by chain, side view.
PDB entry 5to2 coloured by chain, top view.
Source organism: Streptomyces avidinii
Entry authors: Zhang M, Biswas S, Deng W, Yu H

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149690 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptavidin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 125 amino acids
Theoretical weight: 13.05 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 39-163; Coverage: 79%)
Sequence domains: Avidin family
Structure domains: Avidin-like
Streptavidin Chain: D
Molecule details ›
Chain: D
Length: 124 amino acids
Theoretical weight: 12.99 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 39-162; Coverage: 78%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 44.357Å b: 56.738Å c: 57.435Å
α: 87.54° β: 85.89° γ: 67.99°
R-values:
R R work R free
0.206 0.204 0.234
Expression system: Escherichia coli

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