› 5tsg

X-ray diffraction
3.4Å resolution

PilB from Geobacter metallireducens bound to ADP

Released:
DOI: 10.2210/pdb5tsg/pdb
PDB entry 5tsg coloured by chain, front view.
PDB entry 5tsg coloured by chain, side view.
PDB entry 5tsg coloured by chain, top view.
Source organism: Geobacter metallireducens GS-15
Primary publication:
The molecular mechanism of the type IVa pilus motors.
McCallum M, Tammam S, Khan A, Burrows LL, Howell PL
OpenAccess logo Nat Commun 8 15091 (2017)
PMID: 28474682

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial type II secretion system protein E domain-containing protein Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 588 amino acids
Theoretical weight: 64.89 KDa
Source organism: Geobacter metallireducens GS-15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39VU7 (Residues: 1-568; Coverage: 100%)
Gene names: Gmet_1393, pilB
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 3 x ZN
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P3221
Unit cell:
a: 112.12Å b: 112.12Å c: 299.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.274 0.273 0.29
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Type IV pili: dynamics, biophysics and functional consequences.
Craig et al. (2019)
10 more

4 mentions without citation

Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask).
Pelicic V. (2023)
3 more