› 5twx

X-ray diffraction
2.55Å resolution

Crystal Structure of BRD9 bromodomain

Released:
DOI: 10.2210/pdb5twx/pdb
PDB entry 5twx coloured by chain, front view.
PDB entry 5twx coloured by chain, side view.
PDB entry 5twx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Degradation of the BAF Complex Factor BRD9 by Heterobifunctional Ligands.
Remillard D, Buckley DL, Paulk J, Brien GL, Sonnett M, Seo HS, Dastjerdi S, Wühr M, Dhe-Paganon S, Armstrong SA, Bradner JE
Angew Chem Int Ed Engl 56 5738-5743 (2017)
PMID: 28418626

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-190676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 9 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 121 amino acids
Theoretical weight: 13.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H8M2 (Residues: 134-250; Coverage: 20%)
Gene names: BRD9, UNQ3040/PRO9856
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

1 bound ligand:
Ligand 7P7 4 x 7P7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P6522
Unit cell:
a: 75.26Å b: 75.26Å c: 344.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.233 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

33 review citations

Proteolysis-Targeting Chimeras as Therapeutics and Tools for Biological Discovery.
Burslem et al. (2020)
32 more

1 mention without citation

Degradation of the BAF Complex Factor BRD9 by Heterobifunctional Ligands.
Remillard et al. (2017)