5u8t

Electron Microscopy
4.9Å resolution

Structure of Eukaryotic CMG Helicase at a Replication Fork and Implications

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119199 (preferred)
Entry contents:
11 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (12 distinct):
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Minichromosome maintenance protein 5 Chain: 5
DNA replication licensing factor MCM6 Chain: 6
DNA replication licensing factor MCM7 Chain: 7
DNA replication complex GINS protein PSF1 Chain: A
DNA replication complex GINS protein PSF2 Chain: B
DNA replication complex GINS protein PSF3 Chain: C
DNA replication complex GINS protein SLD5 Chain: D
Cell division control protein 45 Chain: E
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: F

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.9Å
Relevant EMDB volumes: EMD-8519
Expression system: Saccharomyces cerevisiae