› 5u8z

X-ray diffraction
1.9Å resolution

Structure of Fe-CAO1 in complex with beta-fluororesveratrol

Released:
DOI: 10.2210/pdb5u8z/pdb
PDB entry 5u8z coloured by chain, front view.
PDB entry 5u8z coloured by chain, side view.
PDB entry 5u8z coloured by chain, top view.
Source organism: Neurospora crassa OR74A
Primary publication:
Structure and Spectroscopy of Alkene-Cleaving Dioxygenases Containing an Atypically Coordinated Non-Heme Iron Center.
Sui X, Weitz AC, Farquhar ER, Badiee M, Banerjee S, von Lintig J, Tochtrop GP, Palczewski K, Hendrich MP, Kiser PD
Biochemistry 56 2836-2852 (2017)
PMID: 28493664

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carotenoid cleavage oxygenase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 526 amino acids
Theoretical weight: 59.5 KDa
Source organism: Neurospora crassa OR74A
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7S860 (Residues: 1-526; Coverage: 100%)
Gene names: NCU07008, cao-1
Sequence domains: Retinal pigment epithelial membrane protein

Ligands and Environments

4 bound ligands:
Ligand FE2 4 x FE2
Ligand 83D 4 x 83D
Ligand CL 4 x CL
Ligand DMS 2 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3221
Unit cell:
a: 101.037Å b: 101.037Å c: 448.622Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.213
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The molecular aspects of absorption and metabolism of carotenoids and retinoids in vertebrates.
Widjaja-Adhi et al. (2020)
4 more

1 mention without citation

Structure and Spectroscopy of Alkene-Cleaving Dioxygenases Containing an Atypically Coordinated Non-Heme Iron Center.
Sui et al. (2017)