› 5ua2

X-ray diffraction
2.9Å resolution

Mycobacterium tuberculosis KstR in complex with a 26-bp DNA operator

Released:
DOI: 10.2210/pdb5ua2/pdb
PDB entry 5ua2 coloured by chain, front view.
PDB entry 5ua2 coloured by chain, side view.
PDB entry 5ua2 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Ho NAT, Dawes SS, Baker EN, Lott JS, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119203 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional repressor KstR Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 24.47 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96856 (Residues: 1-220; Coverage: 100%)
Gene names: Rv3574, kstR
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2
DNA (5'-D(P*CP*CP*CP*AP*CP*TP*AP*GP*AP*AP*CP*GP*TP*GP*TP*TP*CP*TP*AP*AP*TP*AP*GP*T)-3') Chain: M
Molecule details ›
Chain: M
Length: 26 nucleotides
Theoretical weight: 7.99 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
DNA (5'-D(P*AP*CP*TP*AP*TP*TP*AP*GP*AP*AP*CP*AP*CP*GP*TP*TP*CP*TP*AP*GP*TP*GP*GP*G)-3') Chain: N
Molecule details ›
Chain: N
Length: 26 nucleotides
Theoretical weight: 7.99 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 60.365Å b: 79.545Å c: 77.124Å
α: 90° β: 106.81° γ: 90°
R-values:
R R work R free
0.231 0.23 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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