› 5uc0

X-ray diffraction
1.73Å resolution

Crystal Structure of Beta-barrel-like, Uncharacterized Protein of COG5400 from Brucella abortus

Released:
DOI: 10.2210/pdb5uc0/pdb
PDB entry 5uc0 coloured by chain, front view.
PDB entry 5uc0 coloured by chain, side view.
PDB entry 5uc0 coloured by chain, top view.
Source organism: Brucella abortus 2308
Primary publication:
Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus.
Herrou J, Willett JW, Fiebig A, Varesio LM, Czyż DM, Cheng JX, Ultee E, Briegel A, Bigelow L, Babnigg G, Kim Y, Crosson S
Mol Microbiol 111 637-661 (2019)
PMID: 30536925

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-174190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF1134 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 17.39 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2YQA6 (Residues: 39-198; Coverage: 81%)
Gene name: BAB1_1612
Sequence domains: Envelope integrity protein A

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand CL 2 x CL
Ligand 1PE 2 x 1PE
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P63
Unit cell:
a: 113.075Å b: 113.075Å c: 64.308Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.176 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Brucellosis detection and the role of Brucella spp. cell wall proteins.
Bulashev et al. (2023)
1 more

2 mentions without citation

The Brucella Cell Envelope.
Alakavuklar et al. (2023)
1 more