› 5ugh

X-ray diffraction
2.06Å resolution

Crystal structure of Mat2a bound to the allosteric inhibitor PF-02929366

Released:
DOI: 10.2210/pdb5ugh/pdb
PDB entry 5ugh coloured by chain, front view.
PDB entry 5ugh coloured by chain, side view.
PDB entry 5ugh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Targeting S-adenosylmethionine biosynthesis with a novel allosteric inhibitor of Mat2A.
Quinlan CL, Kaiser SE, Bolaños B, Nowlin D, Grantner R, Karlicek-Bryant S, Feng JL, Jenkinson S, Freeman-Cook K, Dann SG, Wang X, Wells PA, Fantin VR, Stewart AE, Grant SK
Nat Chem Biol 13 785-792 (2017)
PMID: 28553945

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase isoform type-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 397 amino acids
Theoretical weight: 43.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P31153 (Residues: 1-395; Coverage: 100%)
Gene names: AMS2, MAT2A, MATA2
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

1 bound ligand:
Ligand 8AJ 4 x 8AJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 99.731Å b: 109.1Å c: 149.012Å
α: 90° β: 100.04° γ: 90°
R-values:
R R work R free
0.177 0.176 0.203
Expression system: Spodoptera frugiperda

Quick links

Citations

14 review citations

Methionine metabolism in health and cancer: a nexus of diet and precision medicine.
Sanderson et al. (2019)
13 more

3 mentions without citation

Control and regulation of S-Adenosylmethionine biosynthesis by the regulatory β subunit and quinolone-based compounds.
Panmanee et al. (2019)
2 more