› 5ugx

X-ray diffraction
2.35Å resolution

Crystal Structure of the Tyrosine Kinase Domain of FGF Receptor 2 Harboring a E565A/D650V double Gain-of-Function Mutation

Released:
DOI: 10.2210/pdb5ugx/pdb
PDB entry 5ugx coloured by chain, front view.
PDB entry 5ugx coloured by chain, side view.
PDB entry 5ugx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Elucidation of a four-site allosteric network in fibroblast growth factor receptor tyrosine kinases.
Chen H, Marsiglia WM, Cho MK, Huang Z, Deng J, Blais SP, Gai W, Bhattacharya S, Neubert TA, Traaseth NJ, Mohammadi M
OpenAccess logo Elife 6 (2017)
PMID: 28166054

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21802 (Residues: 458-768; Coverage: 39%)
Gene names: BEK, FGFR2, KGFR, KSAM
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand ACP 2 x ACP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 66.973Å b: 77.86Å c: 115.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.215 0.275
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, activation and dysregulation of fibroblast growth factor receptor kinases: perspectives for clinical targeting.
Farrell et al. (2018)
3 more

1 mention without citation

Elucidation of a four-site allosteric network in fibroblast growth factor receptor tyrosine kinases.
Chen et al. (2017)