› 5uof

X-ray diffraction
1.7Å resolution

Crystal structure of alpha,alpha-trehalose 6-phosphate sythase from Burkholderia multivorans

Released:
DOI: 10.2210/pdb5uof/pdb
PDB entry 5uof coloured by chain, front view.
PDB entry 5uof coloured by chain, side view.
PDB entry 5uof coloured by chain, top view.
Source organism: Burkholderia multivorans ATCC 17616
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trehalose-6-phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 481 amino acids
Theoretical weight: 53.79 KDa
Source organism: Burkholderia multivorans ATCC 17616
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3KGN3 (Residues: 1-473; Coverage: 100%)
Gene names: BMULJ_02361, otsA
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand EDO 39 x EDO
Ligand SER 1 x SER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 117.58Å b: 128.92Å c: 85.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.185
Expression system: Escherichia coli

Quick links