› 5uqe

X-ray diffraction
3.6Å resolution

Multidomain structure of human kidney-type glutaminase(KGA/GLS)

Released:
DOI: 10.2210/pdb5uqe/pdb
PDB entry 5uqe coloured by chain, front view.
PDB entry 5uqe coloured by chain, side view.
PDB entry 5uqe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The origin and evolution of human glutaminases and their atypical C-terminal ankyrin repeats.
Pasquali CC, Islam Z, Adamoski D, Ferreira IM, Righeto RD, Bettini J, Portugal RV, Yue WW, Gonzalez A, Dias SMG, Ambrosio ALB
J Biol Chem 292 11572-11585 (2017)
PMID: 28526749

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase kidney isoform, mitochondrial 65 kDa chain Chains: A, B, C, D, F
Molecule details ›
Chains: A, B, C, D, F
Length: 520 amino acids
Theoretical weight: 58.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O94925 (Residues: 137-656; Coverage: 78%)
Gene names: GLS, GLS1, KIAA0838
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 04A 3 x 04A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P42212
Unit cell:
a: 144.42Å b: 144.42Å c: 615.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.276 0.274 0.313
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.
Li et al. (2019)
4 more

2 mentions without citation

Glutamine Transport and Mitochondrial Metabolism in Cancer Cell Growth.
Scalise et al. (2017)
1 more