› 5usn

X-ray diffraction
1.9Å resolution

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssRNA/ssDNA chimera (rGrGrUTACGGT)

Released:
DOI: 10.2210/pdb5usn/pdb
PDB entry 5usn coloured by chain, front view.
PDB entry 5usn coloured by chain, side view.
PDB entry 5usn coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Discrimination against RNA Backbones by a ssDNA Binding Protein.
Lloyd NR, Wuttke DS
Structure 26 722-733.e2 (2018)
PMID: 29681468

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113193 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 16.96 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O13988 (Residues: 199-339; Coverage: 25%)
Gene names: SPAC26H5.06, pot1
Sequence domains: ssDNA-binding domain of telomere protection protein
Structure domains: Nucleic acid-binding proteins
1-3R_9mer DNA/RNA (5'-R(*GP*GP*U)-D(P*TP*AP*CP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.82 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 41.64Å b: 59.8Å c: 66.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.183 0.224
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

1 citations in other articles

Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function.
Glustrom et al. (2018)

2 mentions without citation

Discrimination against RNA Backbones by a ssDNA Binding Protein.
Lloyd et al. (2018)
1 more