› 5uvd

X-ray diffraction
1.86Å resolution

Crystal structure of an antigenic nucleotidyltransferase-like protein from Paracoccidioides brasiliensis

Released:
DOI: 10.2210/pdb5uvd/pdb
PDB entry 5uvd coloured by chain, front view.
PDB entry 5uvd coloured by chain, side view.
PDB entry 5uvd coloured by chain, top view.
Source organism: Paracoccidioides brasiliensis Pb18
Primary publication:
Structural and immunological characterization of a new nucleotidyltransferase-like antigen from Paracoccidioides brasiliensis.
Coitinho JB, Costa MAF, Melo EM, Morais EA, de Andrade LGA, da Rocha AM, de Magalhães MTQ, Favaro DC, Bleicher L, Pedroso ERP, Goes AM, Nagem RAP
Mol Immunol 112 151-162 (2019)
PMID: 31108423

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleotidyltransferase-like protein Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.76 KDa
Source organism: Paracoccidioides brasiliensis Pb18
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C1GM11 (Residues: 1-219; Coverage: 100%)
Gene name: PADG_08402
Sequence domains: Nucleotidyl transferase AbiEii toxin, Type IV TA system

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 33.649Å b: 51.105Å c: 121.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.159 0.222
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

One Century of Study: What We Learned about Paracoccidioides and How This Pathogen Contributed to Advances in Antifungal Therapy.
Seki Kioshima et al. (2021)

2 mentions without citation

Antitoxin autoregulation of M. tuberculosis toxin-antitoxin expression through negative cooperativity arising from multiple inverted repeat sequences.
Beck et al. (2020)
1 more