› 5uxb

X-ray diffraction
2.79Å resolution

Crystal structure of macrolide 2'-phosphotransferase MphH from Brachybacterium faecium, apoenzyme

Released:
DOI: 10.2210/pdb5uxb/pdb
PDB entry 5uxb coloured by chain, front view.
PDB entry 5uxb coloured by chain, side view.
PDB entry 5uxb coloured by chain, top view.
Source organism: Brachybacterium faecium DSM 4810
Primary publication:
The evolution of substrate discrimination in macrolide antibiotic resistance enzymes.
Pawlowski AC, Stogios PJ, Koteva K, Skarina T, Evdokimova E, Savchenko A, Wright GD
OpenAccess logo Nat Commun 9 112 (2018)
PMID: 29317655

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111721 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 32.23 KDa
Source organism: Brachybacterium faecium DSM 4810
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7MEP1 (Residues: 1-298; Coverage: 100%)
Gene name: Bfae_22410
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 75.933Å b: 79.741Å c: 99.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The Role of Antibiotic-Target-Modifying and Antibiotic-Modifying Enzymes in Mycobacterium abscessus Drug Resistance.
Luthra et al. (2018)
6 more

1 mention without citation

The evolution of substrate discrimination in macrolide antibiotic resistance enzymes.
Pawlowski et al. (2018)