› 5v0q

X-ray diffraction
2.4Å resolution

Original engineered variant of I-OnuI meganuclease targeting the HIV integrase gene; harbors 49 point mutations relative to wild-type I-OnuI

Released:
DOI: 10.2210/pdb5v0q/pdb
PDB entry 5v0q coloured by chain, front view.
PDB entry 5v0q coloured by chain, side view.
PDB entry 5v0q coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses.
Niyonzima N, Lambert AR, Werther R, De Silva Feelixge H, Roychoudhury P, Greninger AL, Stone D, Stoddard BL, Jerome KR
Protein Eng Des Sel 30 503-522 (2017)
PMID: 28873986

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-119256 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
I-OnuI_e-vHIVInt_v1 Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 35.08 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
DNA (26-MER) Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 8.04 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (26-MER) Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.94 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 39.693Å b: 74.419Å c: 164.861Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.186 0.23
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

CRISPR-Cas9 gene editing of hepatitis B virus in chronically infected humanized mice.
Stone et al. (2021)
1 more

1 mention without citation

Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses.
Niyonzima et al. (2017)