› 5v73

X-ray diffraction
1.68Å resolution

Crystal structure of N110A mutant of human macrophage migration inhibitory factor

Released:
DOI: 10.2210/pdb5v73/pdb
PDB entry 5v73 coloured by chain, front view.
PDB entry 5v73 coloured by chain, side view.
PDB entry 5v73 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pantouris G, Lolis E

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 3 x GOL
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 67.918Å b: 68.253Å c: 86.898Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.175
Expression system: Escherichia coli

Quick links