› 5vhs

Electron Microscopy
8.8Å resolution

Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle

Released:
DOI: 10.2210/pdb5vhs/pdb
PDB entry 5vhs coloured by chain, front view.
PDB entry 5vhs coloured by chain, side view.
PDB entry 5vhs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.
Lu Y, Wu J, Dong Y, Chen S, Sun S, Ma YB, Ouyang Q, Finley D, Kirschner MW, Mao Y
Mol Cell 67 322-333.e6 (2017)
PMID: 28689658
Related structures: EMD-8684

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126188 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (18 distinct):
26S proteasome regulatory subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 39.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P35998 (Residues: 73-424; Coverage: 81%)
Gene names: MSS1, PSMC2
Sequence domains:
26S proteasome regulatory subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 341 amino acids
Theoretical weight: 38.03 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P62191 (Residues: 93-433; Coverage: 78%)
Gene name: PSMC1
Sequence domains:
26S proteasome regulatory subunit 8 Chain: C
26S proteasome regulatory subunit 6B Chain: D
26S proteasome regulatory subunit 10B Chain: E
26S proteasome regulatory subunit 6A Chain: F
26S proteasome non-ATPase regulatory subunit 1 Chain: U
26S proteasome non-ATPase regulatory subunit 3 Chain: V
26S proteasome non-ATPase regulatory subunit 12 Chain: W
26S proteasome non-ATPase regulatory subunit 11 Chain: X
26S proteasome non-ATPase regulatory subunit 6 Chain: Y
26S proteasome non-ATPase regulatory subunit 7 Chain: Z
26S proteasome non-ATPase regulatory subunit 13 Chain: a
26S proteasome non-ATPase regulatory subunit 4 Chain: b
26S proteasome non-ATPase regulatory subunit 14 Chain: c
26S proteasome non-ATPase regulatory subunit 8 Chain: d
26S proteasome complex subunit SEM1 Chain: e
26S proteasome non-ATPase regulatory subunit 2 Chain: f

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 8.8Å
Relevant EMDB volumes: EMD-8684
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Cryo-EM for Small Molecules Discovery, Design, Understanding, and Application.
Scapin et al. (2018)
6 more

1 mention without citation

Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.
Lu et al. (2017)