› 5viv

X-ray diffraction
1.33Å resolution

Crystal structure of monomeric near-infrared fluorescent protein miRFP670

Released:
DOI: 10.2210/pdb5viv/pdb
PDB entry 5viv coloured by chain, front view.
PDB entry 5viv coloured by chain, side view.
PDB entry 5viv coloured by chain, top view.
Source organism: Rhodopseudomonas palustris
Primary publication:
Designing brighter near-infrared fluorescent proteins: insights from structural and biochemical studies.
Baloban M, Shcherbakova DM, Pletnev S, Pletnev VZ, Lagarias JC, Verkhusha VV
OpenAccess logo Chem Sci 8 4546-4557 (2017)
PMID: 28936332

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103069 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytochrome chromophore attachment site domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.53 KDa
Source organism: Rhodopseudomonas palustris
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A161I5N6 (Residues: 1-314; Coverage: 43%)
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand 9UY 1 x 9UY
Ligand 9V1 1 x 9V1
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 52.539Å b: 53.282Å c: 107.397Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.189
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Near-Infrared Fluorescent Proteins: Multiplexing and Optogenetics across Scales.
Shcherbakova et al. (2018)
2 more

6 mentions without citation

Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing.
Oliinyk et al. (2019)
5 more